RNA Sequencing:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
Recombinant:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
Plasmid Preparation:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
Multiple Displacement Amplification:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
Software:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
Targeted Proteomics:Article Title: Metabolic reinvigoration of NK cells by IL-21 enhances immunotherapy against MHC class I-deficient solid tumors.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Bulk RNA-seq data This paper GEO: GSE28973 scRNA-seq data This paper GEO: GSE282121 Oligonucleotides (5 ′ to 3 ′ ) sgLdha-1-TTGGTGAGTATACCTTGGGC This paper N/A sgLdha-2-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-3-GGGACTTGGTGAGTATACCT This paper N/A sgLdha-4-GCACCGGCTAAAGTCACTGT This paper N/A sgScramble-TGCGAATACGCCCACGCGAT This paper N/A Recombinant DNA Plasmid gWIZ_Kozak_mIgGSP_mouse IL-21− Fc This paper N/A Plasmid plenti guide Thy1.1 Addgene RRID: Addgene_128063 Plasmid MSCV puro Addgene RRID: Addgene_68469 Plasmid pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid pCMV-dR8.2 dvpr Addgene RRID: Addgene_8455 Experimental models: Cell lines B16F10_β2m − /− Gift from Prof. David H. Raulet N/A CT26_β2m − /− Gift from Prof. David H. Raulet N/A RMA-S Gift from Prof. Werner Held N/A Phoenix-Eco ATCC Cat # CRL-3214 TM , RRID: CVCL_H717 MDA-MB-231 ATCC Cat # HTB-26 TM RRID: CVCL_0062 U87 ATCC Cat # HTB-14 TM RRID: CVCL_0022 HepG2 Haixing Biosciences Cat # TCH-C196 HCT116 Haixing Biosciences Cat # TCH-C185 Experimental models: Organisms/strains Mouse: C57BL/6J Charles River Laboratories Strain #: 000664 RRID: IMSR_JAX:000664 Mouse: BALB/cByJ Charles River Laboratories Strain #: 001026 RRID: IMSR_JAX:001026 Mouse: B6.SJL-Ptprc a Pepc b /BoyCrl The Jackson Laboratory Strain #: 002014 RRID: IMSR_JAX:002014 Mouse: Gt(ROSA)26Sor tm1.1(CAG-cas9 * ,− EGFP)Fezh /J The Jackson Laboratory Strain #: 024858 RRID: IMSR_JAX:026179 Mouse: NOD-Prkdcscid Il2rgem1/Smoc (M-NSG) Shanghai Model Organisms Center, Inc. Cat NO. NM-NSG-001 RRID: IMSR_NM-NSG-001 Software and algorithms GraphPad Prism 10 https://www.graphpad.com/ RRID:SCR_002798 FlowJo v10 https://www.flowjo.com/flowjo/ overview RRID:SCR_008520 Rstudio v.4.3.1 https://cran.rstudio.com/bin/ windows/base/old/4.3.1/ RRID:SCR_000432 featureCounts Subread package N/A Agilent Masshunter Quantitative Analysis software Version B.07.00 http://www.agilent.com/en-us/ products/software-informatics/ masshunter-suite/masshunter/ masshunter-software RRID:SCR_015040 MRM PROBS Tsugawa et al. 79 N/A Other Amicon® Ultra-15 Centrifugal Filter Unit Merck Millipore Cat # UFC901024 Millex-HV Syringe Filter Unit, 0.22 μm Merck Millipore Cat # SLGSV255F (Continued on next page) 22 Cell Reports 45, 117035, March 24, 2026
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